Pharmacological potential of acamprosate in epilepsy: A study in Drosophila Melanogaster
Mahendra Kumar Sahu, Govind Singh
Epilepsy is the tendency to have seizures that start in the brain. The brain uses electrical signals to pass messages between brain cells. If these signals are disrupted, this can lead to a seizure. Epilepsy models have been developed to appraise the pathophysiology of epileptic seizures and to search for new effective anti-epileptic drugs. Around 50 million people worldwide have epilepsy. Establishment of bang induced seizure model of epilepsy in Drosophila melanogaster in laboratory. When fed to seizure-sensitive Drosophila, In particular, for drug treatments, the recovery time from seizure and paralysis is greatly reduced compared with untreated flies. Intriguingly we find that drug treatments result in a reduction in seizure sensitivity recovery time. Evaluation of pharmacological potential of acamprosate in bang induced seizure model of epilepsy in Drosophila melanogaster. Acamprosate is a synthetic amino acid and a neurotransmitter analogue that is used as an alcohol deterrent in management of alcohol dependence and abuse. Behavior parameters using in this protocol is mean recovery time (MRT), bang sensitivity (BS) and climbing assay. Biochemical parameter using in this protocol is estimation of total protein content, malondialdehyde (MDA) estimation, catalase (CAT) estimation, superoxide dismutase (SOD) estimation and nitric oxide (NO) estimation. Correlation of data observed in Drosophila model.
Mahendra Kumar Sahu, Govind Singh. Pharmacological potential of acamprosate in epilepsy: A study in Drosophila Melanogaster. International Journal of Advanced Research and Development, Volume 1, Issue 10, 2016, Pages 66-72