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Hospitals generate substantial quantity of wastes that has potential to cause health and environmental hazards. In order to streamline the waste collection, processing and disposal practices, the Government of India in 1998 notified rules known as the Biomedical Waste (Management and Handling) Rules, 1998. These Rules were revised from time to time. On March 28, 2016, the Government of India published the Biomedical Waste Management Rules, 2016 in superstation of the Biomedical Waste (Management and Handling) Rules, 1998. In the new Biomedical Waste Management Rules, 2016, several changes and additions have been made to further improve the collection, segregation, processing, treatment and disposal of the biomedical wastes in an environmentally sound manner. The existing biomedical waste treatment facilities need to meet the revised standards of emissions and effluent also to install the continuous monitoring equipments. This review highlights the main changes and additions included in the Biomedical Waste Management Rules, 2016.